atto 550 flow cytometry channel

DC sheath flow detection method Sysmex analysers use the DC sheath flow detection method to count red blood cells and platelets, RBC and PLT. The panel was used for defining T cell subsets using lysed whole blood (BD Pharm Lyse Lysing Buffer) from a normal donor. Low carryover is essential in research applications to ensure sample purity and data integrity. Fluorescence was then read by flow cytometry and the data are presented as a stimulation index SEM computed as follows: MFI of activated cells relative to MFI of resting cells but loaded with DCFDA (Control). Up to 14 parameters from 4 lasers Spectra varies slightly from lot to lot. Flow cytometry was performed using FACSAria IIIu (Becton Dickinson). Antibody conjugation is a critical step in many molecular-biology research assays. Commun. With over 35 years of research, development, and manufac- Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO) is directly labeled with an ATTO-550 fluorescent dye. HTS provides rapid, fully automated sample acquisition from 96- and 384-well microtiter plates. The fluorescence is excited most efficiently in the range 610 645 nm. Y. Li, A. Chukun Li, Q. Xu, Intracellular Delivery of HisTagged GenomeEditing Proteins Enabled by Nitrilotriacetic AcidContaining Lipidoid Nanoparticles, Advanced Healthcare Materials 8 (2019). 0 In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. The fluorescence is excited most efficiently in the 540 - 565 nm range. The light produced by lasers in a flow cytometer is scattered by cells in the sample, measured by detectors, and then translated to signals that can be analyzed and measured. 29, 1282 (2012). D. Daems, W. Pfeifer, I. Rutten, B. Sacc, D. Spasic, J. Lammertyn. Chen, W.-Y. I. Hoffecker, S. Chen, A. Gdin, A. Bosco, A. Teixeira, B. Hgberg, SolutionControlled Conformational Switching of an Anchored Wireframe DNA Nanostructure, Small 15 (2019). 0000002570 00000 n 0000276030 00000 n The website has an accessibility menu. Storage. S. Huo, M. Tabaka, A flexible fluorescence correlation spectroscopy based method for quantification of the DNA double labeling efficiency with precision control, Laser Phys. 0000000016 00000 n A fluorophore free in solution may have a different quantum yield than the same fluorophore attached to a protein, which in turn also depends on the extent of protein-to-fluorophore labeling (1-3). 550/30 TagYFP: 508: 524: 488, 514, 532: . D. Roth, M. Nasir, P. Ginzburg, P. Wang, A. ATTO 550 Orange 554 576 791 Cy3.5 Orange Red 581 596 1,286 microscopy, flow cytometry and immunohistochemistry. However, all three are based on multiple dyes in protein complexes and photobleach very Using flow cytometry analysis, P2Y 1, P2Y 2, P2Y 4, P2Y 6, P2Y 11, P2Y 12, and P2Y 13 receptors were detected in osteoblasts using their respective Alomone Labs antibodies (open histograms). 0000190721 00000 n Store lyophilized or in solution at 4C, desiccated. 0000003664 00000 n hb```b`a`g` l,s&``+Lj9k|ApTGRg\ZZZ@ 0000196491 00000 n Corrie, A. Rhmann, M. Trau, Biomolecular screening with novel organosilica microspheres, Chem. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. R. Ankri, A. Basu, A. Ulku, C. Bruschini, E. Charbon, S. Weiss, X. Michalet, Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium, ACS Photonics 7, 68 (2019). -ATTO 550 product information, - NHS-ester Lett. A one parameter histogram plotting channel number vs. number of events. S. Mukherjee, J.-M. Knop, R. Oliva, S. Mbitz, R. Winter, Untangling the interaction of -synuclein with DNA i-motifs and hairpins by volume-sensitive single-molecule FRET spectroscopy, RSC Chemical Biology 2, 1196 (2021). E. J. 0000196962 00000 n Provisional Application No. Technical Summary. Andy Fluor Dyes: Excellent Photostability. M. Zoppo, N. Okoniewski, S. Pantelyushin, J. Vom Berg, K. Schirmer, A ribonucleoprotein transfection strategy for CRISPR/Cas9mediated gene editing and single cell cloning in rainbow trout cells, Cell & Bioscience 11, 1 (2021). Antibodies allow selective detection of specific proteins. 19% tax excl. H. Mnck, D. Toppe, E. Michael, S. Sigrist, V. Richter, D. Hilpert, D. Raccuglia, M. Efetova, M. Schwrzel. Fluorescence Spectrum Analyzer for Flow Cytometry Use this spectral viewer tool to compare fluorescent spectra excitation by different lasers and emission into different bandpass filters as an aid to multicolor flow cytometry panel design. G. Lin, M. Lewandowska, Plasmon-enhanced fluorescence provided by silver nanoprisms for sensitive detection of sulfide, Sensors and Actuators B: Chemical 292, 241 (2019). Second, to optically distinguish and quantify intracellular cholesterol accumulation, we have adapted the classical filipin cholesterol staining protocol. Phone: 305-822-0666 Ability to navigate with the keyboard Kim, W. Sung, N. Lee, Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks, Angewandte Chemie International Edition 54, 8943 (2015). Irving et al., Reactive centre loop mutants of -1-antitrypsin reveal position-specific effects on intermediate formation along the polymerization pathway, Biosci. Converse Library Sample, S. Yeou, N. Lee, Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force, Bulletin of the Korean Chemical Society 42, 1151 (2021). startxref Northland College Women's Hockey Roster, J. Wardyn, A. Chan, A. Jeyasekharan, A Robust Protocol for CRISPR-Cas9 Gene Editing in Human Suspension Cell Lines, Current Protocols 1, e286 (2021). 0000005582 00000 n Spectral flow cytometry, an emerging methodology that is not confined by the "one color, one detector" paradigm, shows promise in the facile detection of multiple fluorescent proteins. This automatic decision help to standardise and streamline your entire platelet workflow. Telefon: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: orders@antikoerper-online.de ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. To view a known dye, scroll down the menu on the left and click to select. Sc., President and Technical Director, Omega Optical is a leader in photonics, Expression of KV1.5 in mouse cerebellum - Immunohistochemical staining of perfusion fixed free-floating frozen mouse brain sections usingAnti-KV1.5 (KCNA5)-ATTO-550 Antibody(#APC-004-AO) (1:50) (red). Lo, F. Emran, I. Kays, X.-J. T. Munmun, A. Kabir, K. Sada, A. Kakugo, Complete, rapid and reversible regulation of the motility of a nano-biomolecular machine using an osmolyte trimethylamine-N-oxide, Sensors and Actuators B: Chemical 304, 127231 (2020). The antibody ships as a lyophilized powder at room temperature. A. Silvestri, D. Di Silvio, I. Llarena, R. Murray, M. Marelli, L. Lay, L. Polito, S. Moya, Influence of surface coating on the intracellular behaviour of gold nanoparticles, Nanoscale 9, 14730 (2017). As expected, the addition of the amphiphile triggered the appearance of fluorescent pixels in the red channel of the confocal fluorescent microscopy images (Fig. Y. Cheng, T. Stakenborg et al., Fluorescence Near Gold Nanoparticles for DNA Sensing, Anal. ATTO 594 exhibits excellent water solubility, very good stability over . R. Schoch, I. Barel, F. Brown, G. Haran, Lipid diffusion in the distal and proximal leaflets of supported lipid bilayer membranes studied by single particle tracking, The Journal of Chemical Physics 148, 123333 (2018). 0000038853 00000 n 2 Images : +351 30 8808 050 Fax : +351 30 8808 052 info@quimigen.pt www.quimigen.pt Expression of TRPV4 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.A D. Staining usingAnti-TRPV4Antibody (#ACC-034) (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.B E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.C. 0000128088 00000 n All transmission and blocking (OD) data are actual, measured spectra of representative production lots. We are continuing our efforts to enhance the accessibility of the website as much as possible, out of our moral obligation to enable the use of the website for the population as a whole, including people with disabilities. 0000196280 00000 n Atto 550 is an alternative to rhodamine dyes, Cy3, and Alexa Fluor 550, and flow cytometry. Increasing and decreasing the website fonts A (-) in a table cell represents no applicable spillover. It includes an automated sheath and waste fluid control system that reduces daily maintenance by incorporating two 20-L containers (Cubitainers), Fluidic sensors maintain constant pressure, while a fluidics monitoring system warns when sheath fluid is low or empty or when the waste container is full. 0000005723 00000 n This website is run by the accessibility program of the "Accessible with a Click" company and is run via a designated accessibility server. Flow Cytometry. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Tomov, R. Tsukanov et al., Rational Design of DNA Motors: Fuel Optimization through Single-Molecule Fluorescence, J. 0000007049 00000 n B. Hellenkamp, S. Schmid, O. Doroshenko, O. Opanasyuk, R. Khnemuth, S. Rezaei Adariani, B. Ambrose, M. Aznauryan, A. Barth, V. Birkedal, M. Bowen, H. Chen, T. Cordes, T. Eilert, C. Fijen, C. Gebhardt, M. Gtz, G. Gouridis, E. Gratton, T. Ha, P. Hao, C. Hanke, A. Hartmann, J. Hendrix, L. Hildebrandt, V. Hirschfeld, J. Hohlbein, B. Hua, C. Hbner, E. Kallis, A. Kapanidis, J.-Y. N. Gilat, D. Torchinsky, S. Margalit, Y. Michaeli, S. Avraham, H. Sharim, N. Elkoshi, C. Levy, S. Zirkin, Y. Ebenstein, Rapid Quantification of Oxidation and UV Induced DNA Damage by Repair Assisted Damage Detection-(Rapid RADD), Analytical Chemistry 92, 9887 (2020). Chem. 0000214142 00000 n 0000186734 00000 n Spark Blue 550, Spark NIR 685) are only recommended for use in multicolor spectral flow cytometry panels. Products are lyophilized and ship at room temperature - FREE OF CHARGE. The BD Special Order Research Product program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Converse Library Sample, 9 0 obj <> endobj Ffx Qactuar Monster Arena, 100 nm.4346 Flow cytometry in combination with Fluorescently labelled ATTO 550-DOPE was purchased from Atto-Tec GmbH (Siegen, Germany). ATTO-590. ATTO-550 (554/576) and ATTO-620 channel. M. Chinnaraj, D. Barrios, C. Frieden, T. Heyduk, R. Flaumenhaft, N. Pozzi, Bioorthogonal Chemistry Enables Single-Molecule FRET Measurements of Catalytically Active Protein Disulfide Isomerase, Encyclopedia of Analytical Chemistry 22, 134 (2021). 42, 252 (2012). CF488A Dye It consists in the uptake of pathogenic or cellular targets larger than 0.5 m. P. Ghenuche, J. de Torres et al., Nanophotonic Enhancement of the Forster Resonance Energy-Transfer Rate with Single Nanoapertures, Nano Lett. Surawski, B.J. 0000017940 00000 n Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates The dye is moderately hydrophilic. 0000213898 00000 n We ship worldwide within 24 hours of our next business day. 2023 BD. Ffx Qactuar Monster Arena, Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN. Underlining links throughout the website. A menu will appear below the graph display with common generic lasers displayed on the left. Spectra Viewer Select machine + Add Fluorophore Fluorophores Ex. This dye is highly suitable for direct flow cytometry (FACS) using the He:Ne laser. Antibody concentration after reconstitution. Phagocytosis by macrophages represents a fundamental process essential for both immunity and tissue homeostasis. HlWI$7+>0z@ARAK ~IFdU4.!Sc)IS 13 ~_\ /I G-r,qS^V(s5OqpTFZg^}wHI)e&aO(RKLp_IH!A` ZlP Rp2bw )Ps9{ Orange fluorescence for microscopy in the Cy3 channel or flow cytometry in the R-PE channel: NucView 530 Caspase-3 Substrate, 1 mM in PBS: 10408: NucView 530 substrate in PBS, for DMSO . Title: Fluorochromes Brightness Chart Author: Research Flow Cytometry Core Subject: Fluorochromes Brightness Chart If the filters are used to screen out all light other than that measured at the maximum absorbance via channel A (Figure 9), FITC will appear green. 0000020039 00000 n Starbound Weapon Tiers, X. Hopkins, W. Gill, R. Kringel, G. Wang, J. Hass, S. Acharya, J. ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B, the commercial alternative to NEDTM. The choice currently selected will be highlighted in blue. If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it.

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